ABSTRACT
The possible interference of resistant pests populations to insecticides in natural enemies in the action thas not been clarified yet. Thus, this study aimed to evaluate Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae) performance on Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) eggs with resistance frequency to the Metaflumizone over six generations of product exposure. Egg cards (2.0 x 7.0 cm) containing eggs from two populations of S. frugiperda, (resistant to Metaflumizone and the other susceptible), were exposed to T. pretiosum females for 24 hours in free-choice and no-choice testing in three generations (G1, G4, and G6). A completely randomized experimental design was used with 25 replications, each consisting of an egg card (experimental unit) containing 20 eggs. The parameters evaluated were: parasitism (%), emergence (%), sex ratio, number of emerged parasitoids per egg and males/females longevity. ANOVA and Tukey test (P≤ 0.05) were applied on the results. Results showed a reduction in parasitism [41.0% (G1) and 28.4% (G4)], egg emergence (17.5%) and parasitoids/egg [16.2 (G4) and 17.2 (G6)] in eggs originating from the population with resistance frequency. Females emerging from G6 populations eggs without exposure to Metaflumizone had greater longevity (3.5 days more) than the resistant population. The sex ratio and male longevity were not affected. The results indicate a reduction in T. pretiosum activity if S. frugiperda populations have some frequency of resistance to Metaflumizone.
A possível interferência de populações de pragas resistentes na ação de inimigos naturais ainda não foi esclarecida. Assim, este trabalho teve como objetivo avaliar o desempenho de Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae) em ovos de Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) com frequência de resistência à Metaflumizona ao longo de seis gerações de exposição ao produto. Cartelas (2,0 x 7,0 cm) com ovos de duas populações de S. frugiperda, (resistente à Metaflumizona e outra suscetível), foram expostas às fêmeas de T. pretiosum por 24 horas em condições de livre escolha e sem chance de escolha por três gerações (G1, G4 e G6). O delineamento experimental foi inteiramente casualizado com 25 repetições, sendo cada repetição composta por uma cartela (unidade experimental) contendo 20 ovos. Os parâmetros avaliados foram: parasitismo (%), emergência (%), razão sexual, número de parasitoides emergidos por ovo e longevidade de machos e fêmeas. ANOVA e teste de Tukey (P≤ 0,05) foram aplicados aos dados coletados. Os resultados mostraram redução do parasitismo [41,0% (G1) e 28,4% (G4)], emergência de ovos (17,5%) e parasitoides/ovo [16,2 (G4) e 17,2 (G6)] em ovos oriundos da população com frequência de resistência. As fêmeas emergidas de ovos da população G6 sem exposição à Metaflumizona, tiveram maior longevidade (3,5 dias a mais) do que a população exposta ao inseticida. A razão sexual e a longevidade de machos não foram afetadas. Os resultados indicam uma redução na atividade de T. pretiosum se as populações de S. frugiperda apresentarem alguma frequência de resistência à Metaflumizona.
Subject(s)
Animals , Hymenoptera/drug effects , Insecticides/administration & dosage , Spodoptera/parasitologyABSTRACT
Trichogramma pretiosum Riley and Trichogrammatoidea annulata De Santis are commonly found in avocado and persimmon orchards in northern Parana state. However, their abundance depends on whether insecticides are used or not to control the key lepidopteran pests Stenoma catenifer (Wals.) (Lepidoptera: Elachistidae) and Hypocala andremona (Stoll) (Lepidoptera: Noctuidae), respectively. The aim of this work was to evaluate the effects of an aqueous neem seed extract (ANSE) at 15, 3 and 1.5 percent, and of an emulsifiable concentrate neem oil (ECNO) at 2.5, 0.5 and 0.25 percent on lifetime parameters of these trichogrammatids as a way of testing the feasibility of integrating the biological and chemical control methods. Chemicals were applied on Anagasta kuehniella (Zeller) (Lepidoptera: Pyralidae) eggs before or after parasitization (one, three or five days). ANSE was more deleterious to both parasitoid species than ECNO, regardless of the concentration and the time of application. The chemicals acted on a concentration and time dependent manner. Treating the host with neem before parasitism was less deleterious to wasp emergence, especially for T. annulata. Pre-treatments (24h) of the host eggs with ECNO at concentrations varying from 0.5 percent to 0.25 percent did not affect T. pretiosum longevity, but 2.5 percent reduced T. annulata survival. Feeding wasps with honey mixed with 0.25 percent ECNO negatively affected T. annulata survival.
Subject(s)
Animals , Azadirachta , Glycerides/pharmacology , Hymenoptera/drug effects , Pest Control, Biological/methods , Plant Extracts/pharmacology , Terpenes/pharmacologyABSTRACT
A strain of T. chilonis, an egg parasitoid of lepidopteran pests tolerant to the most commonly used cyclodiene insecticide--endosulfan was developed in the laboratory. Tolerance to endosulfan was induced by exposing adult parasitoids sequentially from a sub-lethal concentration (0.004%) to the field recommended concentration (0.09%). The strain acquired tolerance to the insecticide after 341 generation of continuous exposure with LC50 values of 1074.96 ppm as compared to LC50 of (70.91 ppm) in susceptible strain. The genetical study showed that F1 crosses exhibited a semi-dominant response to endosulfan with degree of dominance value (D) of 0.58. The resistant factor of tolerant strain was 15.1 folds and of F1 cross were 8.53 folds over susceptible strain. Under net house conditions, the tolerant strain parasitised 56% Helicoverpa armigera eggs on potted cotton plants immediately after an insecticide spray, compared to 3% by the susceptible strain. High percentage survival of the immature stages of the tolerant strain proved their ability to withstand the insecticide load. Breakdown of insecticide tolerance in the strain occurred after four generations in absence of insecticide load. Use of the tolerant strain as a component of bio-intensive IPM in various crops where insecticide use is higher is discussed.
Subject(s)
Animals , Crosses, Genetic , Endosulfan/pharmacology , Hymenoptera/drug effects , Insecticide Resistance/genetics , Insecticides/pharmacology , Lepidoptera/parasitology , Ovum/parasitology , Pest Control, BiologicalABSTRACT
Este trabalho objetivou avaliar a susceptibilidade em condições de laboratório de adultos do parasitóide de ovos Trichogramma pretiosum Riley a fungicidas utilizados na Produção Integrada da Maçã (PIM). Os bioensaios foram conduzidos utilizando-se protocolos padrões da Organização Internacional para o Controle Biológico (IOBC), Seção Regional Paleártica Oeste (WPRS). Foram testados doze fungicidas nas respectivas doses (g ou ml ingrediente ativo/100 L) captana 1 (0,115), captana 2 (0,120), cresoxim-metílico (0,010), enxofre 1 (AG) (0,480), enxofre 2 (0,480), folpete (0,105), mancozebe (0,160), piraclostrobina (0,010), tebuconazol (0,010), tetraconazol (0,005), tiofanato-metílico (0,050) e triforina (0,024). Adicionalmente, utilizou-se água destilada como testemunha negativa e o inseticida triclorfom (0,150) como testemunha positiva. Adultos do parasitóide foram expostos a resíduos dos tratamentos depositados sobre placas de vidro, sendo avaliadas as reduções no parasitismo em relação à testemunha (somente água). Cada tratamento teve quatro repetições. Os resultados permitiram classificar os fungicidas quanto ao impacto sobre os parasitóides em quatro classes: 1, inócuo (< 30 por cento); 2, levemente nocivo (30-79 por cento); 3, moderadamente nocivo (80-99 por cento); e 4, nocivo (> 99 por cento). Dentre os fungicidas testados, 75 por cento foram considerados seletivos (classes 1 e 2) ao parasitóide. Os fungicidas captana 1, captana 2, cresoxim-metílico, folpete, piraclostrobina, tebuconazol, tiofanato-metílico e triforina foram inócuos; mancozebe foi levemente nocivo; enxofre 1 (AG) e tetraconazol foram moderadamente nocivos e enxofre 2 foi nocivo. Estes resultados devem ser levados em consideração quando utilizamos fungicidas no controle de doenças fúngicas em pomares de macieira, preservando assim, o parasitóide de ovos T. pretiosum.
Subject(s)
Animals , Antifungal Agents/pharmacology , Hymenoptera/drug effects , Malus , Pest Control, Biological , Plant Diseases , AgricultureABSTRACT
Effect of solvent residues of Vitex negundo L. and Cassia fistula L. leaves (0.5 and 1%) was studied on egg laying and adult emergence of Callosobruchus maculatus Fab. and on percentage of larval parasitism by Dinarmus vagabundus (Timberlake). Cowpea seeds treated with methanol and aqueous extract of Vitex, at these concentrations (0.5 and 1%), significantly reduced the number of eggs and emergence of F1 adults of C. maculatus. Both Vitex and Cassia extracts did not affect the percentage of parasitism by D. vagabundus on C. maculatus grubs.